Report for: Bioluminescence

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Chapter title	Bioluminescence
Chapter number	21
Book title	Bioluminescence
Published in	Methods in molecular biology, January 2016
DOI	10.1007/978-1-4939-3813-1_21
Pubmed ID	27424911
Book ISBNs	978-1-4939-3811-7, 978-1-4939-3813-1
Authors	Knol-Blankevoort, Vicky T, Mezzanotte, Laura, Rabelink, Martijn J W E, Löwik, Clemens W G M, Kaijzel, Eric L, Vicky T. Knol-Blankevoort, Laura Mezzanotte, Martijn J. W. E. Rabelink, Clemens W. G. M. Löwik, Eric L. Kaijzel Ph.D., Eric L. Kaijzel
Editors	Sung Bae Kim
Abstract	Here we describe a novel multicolor bioluminescent imaging platform that enables us to simultaneously investigate transcription factor nuclear factor-κB (NF-κB) signalling and apoptosis. We genetically modified the human breast cancer cell line MDA-MB-231 to express green, red, and blue light-emitting luciferases to monitor cell number and viability, NF-κB promoter activity, and to enable specific cell sorting and detection, respectively. Z-DEVD-animoluciferin, the pro-luciferin substrate, was used to determine apoptotic caspase 3/7 activity. We used this multicolored cell line for the in vitro evaluation of natural compounds and in vivo optical imaging of tumor necrosis factor (TNFα)-induced NF-κB activation (Mezzanotte et al., PLoS One 9:e85550, 2014).

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.

Geographical breakdown

Country	Count	As %
Unknown	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	16	100%

Demographic breakdown

Readers by professional status	Count	As %
Other	2	13%
Student > Ph. D. Student	2	13%
Professor > Associate Professor	2	13%
Student > Master	2	13%
Student > Bachelor	1	6%
Other	3	19%
Unknown	4	25%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	2	13%
Nursing and Health Professions	2	13%
Agricultural and Biological Sciences	2	13%
Immunology and Microbiology	2	13%
Medicine and Dentistry	2	13%
Other	2	13%
Unknown	4	25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2016.

All research outputs

#20,335,770

of 22,880,691 outputs

Outputs from Methods in molecular biology

#9,921

of 13,133 outputs

Outputs of similar age

#330,743

of 393,699 outputs

Outputs of similar age from Methods in molecular biology

#1,054

of 1,471 outputs

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So far Altmetric has tracked 13,133 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.

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Bioluminescence

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Geographical breakdown

Demographic breakdown

Mendeley readers

Geographical breakdown

Demographic breakdown

Attention Score in Context