You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Timeline
Mendeley readers
Chapter title |
Method to Measure Ubiquitination of NLRs.
|
---|---|
Chapter number | 16 |
Book title |
NLR Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3566-6_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
Authors |
Pablo Palazón-Riquelme, Gloria López-Castejón |
Editors |
Francesco Di Virgilio, Pablo Pelegrín |
Abstract |
Posttranslational modifications are crucial in determining the functions of proteins in the cell. Modification of the NLRP3 inflammasome by the ubiquitin system has recently emerged as a new level of regulation of the inflammasome complex. Here, we describe a method to detect polyubiquitination of NRLP3 using two different approaches: (1) detection with an ubiquin antibody or (2) using TUBE (Tandem Ubiquitin Binding entities). This approach can be used to detect ubiquitination of other NLR or other components of the inflammasome. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 43% |
Lecturer | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Lecturer > Senior Lecturer | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Immunology and Microbiology | 2 | 29% |
Medicine and Dentistry | 2 | 29% |
Unknown | 1 | 14% |