Chapter title |
Measuring IL-1β Processing by Bioluminescence Sensors II: The iGLuc System.
|
---|---|
Chapter number | 6 |
Book title |
NLR Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3566-6_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
Authors |
Eva Bartok, Maria Kampes, Veit Hornung |
Editors |
Francesco Di Virgilio, Pablo Pelegrín |
Abstract |
Inflammasomes are multimeric protein complexes that proteolytically activate caspase-1, which subsequently matures cytokines of the IL-1 family and initiates the induction of pyroptotic cell death. Although this process is central both to pathogen defense and sterile inflammatory processes, there is currently no standard readout available for inflammasome activation which would be suitable for high-throughput applications. We have recently developed a new method for measuring inflammasome activation via the use of a novel proteolytic reporter iGLuc, an IL-1β Gaussia luciferase (iGLuc) fusion protein. Here, we provide detailed protocols for the use of iGLuc in transiently transfected or stably transduced cell lines. Using these protocols, IL-1β maturation as the result of inflammasome activation or other processes can be indirectly measured via the gain of Gaussia luciferase activity of cleaved iGLuc, allowing for rapid inflammasome reconstitution assays and high-throughput screening of inflammasome activity. |
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