| Chapter title |
Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System.
|
|---|---|
| Chapter number | 7 |
| Book title |
NLR Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3566-6_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
| Authors |
Francesco Di Virgilio, Paolo Pinton, Simonetta Falzoni |
| Editors |
Francesco Di Virgilio, Pablo Pelegrín |
| Abstract |
Inflammation is the key pathophysiological response triggered by noxious agents in multicellular organisms. Central to inflammation is detection of exogenous or endogenous danger signals by immune cells. Extracellular ATP is a ubiquitous danger signal released during septic or sterile inflammation. The development of reliable techniques to measure extracellular ATP in vivo has become an urgent need in inflammation studies after the discovery that the most potent plasma membrane receptor responsible for NLRP3 inflammasome activation is an ATP-activated receptor, P2RX7. Here we describe an easy bioluminescence technique for the measurement of extracellular ATP in vivo. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 24% |
| Student > Bachelor | 3 | 14% |
| Student > Doctoral Student | 2 | 10% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 24% |
| Immunology and Microbiology | 3 | 14% |
| Medicine and Dentistry | 3 | 14% |
| Materials Science | 1 | 5% |
| Unknown | 9 | 43% |