↓ Skip to main content

NLR Proteins

Overview of attention for book
Cover of 'NLR Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 NLR Proteins
  3. Altmetric Badge
    Chapter 2 Atypical Inflammasomes.
  4. Altmetric Badge
    Chapter 3 NLR Proteins
  5. Altmetric Badge
    Chapter 4 Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.
  6. Altmetric Badge
    Chapter 5 NLR Proteins
  7. Altmetric Badge
    Chapter 6 Measuring IL-1β Processing by Bioluminescence Sensors II: The iGLuc System.
  8. Altmetric Badge
    Chapter 7 Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System.
  9. Altmetric Badge
    Chapter 8 Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking.
  10. Altmetric Badge
    Chapter 9 NLR Proteins
  11. Altmetric Badge
    Chapter 10 NLR Proteins
  12. Altmetric Badge
    Chapter 11 Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity of Inflammasome Components.
  13. Altmetric Badge
    Chapter 12 Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay.
  14. Altmetric Badge
    Chapter 13 Assessing Caspase-1 Activation.
  15. Altmetric Badge
    Chapter 14 Cell-Free Assay for Inflammasome Activation.
  16. Altmetric Badge
    Chapter 15 Functional Reconstruction of NLRs in HEK293 Cells.
  17. Altmetric Badge
    Chapter 16 Method to Measure Ubiquitination of NLRs.
  18. Altmetric Badge
    Chapter 17 Cytofluorometric Quantification of Cell Death Elicited by NLR Proteins.
  19. Altmetric Badge
    Chapter 18 NLR in Human Diseases: Role and Laboratory Findings.
  20. Altmetric Badge
    Chapter 19 Erratum to: Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence
Attention for Chapter 15: Functional Reconstruction of NLRs in HEK293 Cells.
Altmetric Badge

Readers on

mendeley
10 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Functional Reconstruction of NLRs in HEK293 Cells.
Chapter number 15
Book title
NLR Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3566-6_15
Pubmed ID
Book ISBNs
978-1-4939-3564-2, 978-1-4939-3566-6
Authors

Vincent Compan, Gloria López-Castejón

Editors

Francesco Di Virgilio, Pablo Pelegrín

Abstract

Inflammasomes are molecular complexes that initiate innate immune response. They are mainly expressed by immune cells; however, molecular manipulations in these cells remain very difficult. Here, we describe a simple protocol to overexpress and activate functional NRLP3 inflammasomes in HEK293 cells.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Professor 2 20%
Student > Doctoral Student 1 10%
Student > Ph. D. Student 1 10%
Lecturer 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 20%
Immunology and Microbiology 2 20%
Psychology 1 10%
Agricultural and Biological Sciences 1 10%
Unknown 4 40%