| Chapter title |
Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity of Inflammasome Components.
|
|---|---|
| Chapter number | 11 |
| Book title |
NLR Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3566-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
| Authors |
Catrin Youssif, Bárbara Flix, Olivia Belbin, Mònica Comalada |
| Editors |
Francesco Di Virgilio, Pablo Pelegrín |
| Abstract |
Intracellular signaling and cellular activation have been demonstrated to reside on multi-protein complexes rather than in isolated proteins. Consequently, techniques to resolve these complexes have gained much attention over the last few years. Förster Resonance Energy Transfer (FRET) coupled with Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful tool to discriminate direct interactions between two proteins within a multi-protein complex. Here, we present the use of FRET-FLIM as an experimental tool for the interpretation of the inflammasome composition. We also introduce some considerations required for the correct use of this technique and the control experiments that should be implemented. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 27% |
| Researcher | 3 | 27% |
| Student > Ph. D. Student | 2 | 18% |
| Student > Doctoral Student | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Neuroscience | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Business, Management and Accounting | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 2 | 18% |