Chapter title |
Cytofluorometric Quantification of Cell Death Elicited by NLR Proteins.
|
---|---|
Chapter number | 17 |
Book title |
NLR Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3566-6_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
Authors |
Valentina Sica, Gwenola Manic, Guido Kroemer, Ilio Vitale, Lorenzo Galluzzi |
Editors |
Francesco Di Virgilio, Pablo Pelegrín |
Abstract |
Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands. |
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