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NLR Proteins

Overview of attention for book
Cover of 'NLR Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 NLR Proteins
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    Chapter 2 Atypical Inflammasomes.
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    Chapter 3 NLR Proteins
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    Chapter 4 Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.
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    Chapter 5 NLR Proteins
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    Chapter 6 Measuring IL-1β Processing by Bioluminescence Sensors II: The iGLuc System.
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    Chapter 7 Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System.
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    Chapter 8 Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking.
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    Chapter 9 NLR Proteins
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    Chapter 10 NLR Proteins
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    Chapter 11 Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity of Inflammasome Components.
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    Chapter 12 Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay.
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    Chapter 13 Assessing Caspase-1 Activation.
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    Chapter 14 Cell-Free Assay for Inflammasome Activation.
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    Chapter 15 Functional Reconstruction of NLRs in HEK293 Cells.
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    Chapter 16 Method to Measure Ubiquitination of NLRs.
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    Chapter 17 Cytofluorometric Quantification of Cell Death Elicited by NLR Proteins.
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    Chapter 18 NLR in Human Diseases: Role and Laboratory Findings.
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    Chapter 19 Erratum to: Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence
Attention for Chapter 4: Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.
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Chapter title
Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging.
Chapter number 4
Book title
NLR Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3566-6_4
Pubmed ID
27221482
Book ISBNs
978-1-4939-3564-2, 978-1-4939-3566-6
Authors

Catherine Diamond, James Bagnall, David G. Spiller, Michael R. White, Alessandra Mortellaro, Pawel Paszek, David Brough

Editors

Francesco Di Virgilio, Pablo Pelegrín

Abstract

The pro-inflammatory cytokine interleukin (IL)-1β is an important mediator of the inflammatory response. In order to perform its role in the inflammatory cascade, IL-1β must be secreted from the cell, yet it lacks a signal peptide that is required for conventional secretion, and the exact mechanism of release remains undefined. Conventional biochemical methods have limited the investigation into the processes involved in IL-1β secretion to population dynamics, yet heterogeneity between cells has been observed at a single-cell level. Here, greater sensitivity is achieved with the use of a newly developed vector that codes for a fluorescently labelled version of IL-1β. Combining this with real-time single-cell confocal microscopy using the methods described here, we have developed an effective protocol for investigating the mechanisms of IL-1β secretion and the testing of the hypothesis that IL-1β secretion requires membrane permeabilisation.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 17%
Student > Bachelor 2 17%
Unspecified 1 8%
Other 1 8%
Lecturer 1 8%
Other 2 17%
Unknown 3 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 33%
Agricultural and Biological Sciences 2 17%
Neuroscience 2 17%
Economics, Econometrics and Finance 1 8%
Unspecified 1 8%
Other 0 0%
Unknown 2 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2016.
All research outputs
#15,380,162
of 22,880,691 outputs
Outputs from Methods in molecular biology
#5,350
of 13,132 outputs
Outputs of similar age
#230,990
of 393,712 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,471 outputs
Altmetric has tracked 22,880,691 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,132 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,712 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.

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