Chapter title |
NLR Proteins
|
---|---|
Chapter number | 10 |
Book title |
NLR Proteins
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3566-6_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3564-2, 978-1-4939-3566-6
|
Authors |
Martín-Sánchez, Fátima, Compan, Vincent, Pelegrín, Pablo, Fátima Martín-Sánchez, Vincent Compan, Pablo Pelegrín |
Editors |
Francesco Di Virgilio, Pablo Pelegrín |
Abstract |
Bioluminescent resonance energy transfer (BRET) is a natural phenomenon resulting from a non-radiative energy transfer between a bioluminescent donor (Renilla luciferase) and a fluorescent protein acceptor. BRET signal is dependent on the distance and the orientation between the donor and the acceptor and could be used to study protein-protein interactions and conformational changes within proteins in real time in living cells. This protocol describes the use of BRET technique to study NLRP3 oligomerization in living cells before and during NLRP3 inflammasome activation. |
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