Chapter title |
T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
|
---|---|
Chapter number | 43 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_43 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Leehan, Kerry M, Koelsch, Kristi A, Kerry M. Leehan, Kristi A. Koelsch |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
The ELISPOT is a powerful functional assay used to detect biological activity and immunological secretions from immune cells. In this chapter, we specifically discuss T cell ELISPOT methods for the detection of secreted cytokines. A detailed protocol is given enabling the detection of interferon gamma-secreting CD8(+) T cells and/or peripheral blood mononuclear cells following their isolation and polyclonal activation. Included is a brief discussion on choosing the activation method for your T cell ELISPOT assay, as well as additional instructions for the adaptation of this protocol for the study of memory and antigen-specific T cell responses. |
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