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Mendeley readers
Chapter title |
Western Blotting: Remembrance of Things Past
|
---|---|
Chapter number | 3 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
W. Neal Burnette Ph.D., W. Neal Burnette |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
Western blotting sprung from the need to develop a sensitive visual assay for the antigen specificity of monoclonal antibodies. The technique employed SDS-PAGE of protein antigens, electrophoretic replica transfer of gel-resolved proteins to unmodified nitrocellulose sheets, probing the immobilized antigens with hybridomas, and detection of antibody-antigen complexes with radiolabeled staphylococcal protein A and autoradiography. The simplicity and relevance of the method have led to its expansive application as an immunodiagnostic and a ubiquitous research tool in biology and medicine. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Student > Master | 1 | 8% |
Researcher | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Other | 0 | 0% |
Unknown | 7 | 58% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Agricultural and Biological Sciences | 2 | 17% |
Business, Management and Accounting | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 6 | 50% |