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Mendeley readers
Chapter title |
Immunodetection of P-Selectin Using an Antibody to Its C-Terminal Tag
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Chapter number | 31 |
Book title |
Western Blotting
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2694-7_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
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Authors |
Padmaja Mehta-D’souza |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
P-selectin is a multi-domain glycoprotein expressed on activated endothelial cells and activated platelets. We previously expressed a recombinant form of P-selectin containing only its N-terminal lectin and EGF domains in CHO-K1 cells and showed that these two domains are sufficient to mediate ligand binding. We have now expressed the same construct in CHO-Lec1 cells that make truncated glycans. The uniform glycosylation in these cells should make it easier to crystallize this protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 100% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 100% |