You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
|
---|---|
Chapter number | 30 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Marion L. Greaser Ph.D., Chad M. Warren M.S., Marion L. Greaser, Chad M. Warren |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
Proteins larger than 200 kDa are difficult to separate electrophoretically using polyacrylamide gels, and their transfer during western blotting is typically incomplete. A vertical SDS agarose gel system was developed that has vastly improved resolving power for very large proteins. Complete transfer of proteins as large as titin (Mr 3,000-3,700 kDa) onto blots can be achieved. The addition of a sulfhydryl reducing agent in the upper reservoir buffer and transfer buffer markedly improves the blotting of large proteins. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Lecturer | 20 | 35% |
Student > Bachelor | 9 | 16% |
Student > Master | 6 | 11% |
Lecturer > Senior Lecturer | 2 | 4% |
Student > Ph. D. Student | 1 | 2% |
Other | 3 | 5% |
Unknown | 16 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Nursing and Health Professions | 25 | 44% |
Biochemistry, Genetics and Molecular Biology | 4 | 7% |
Agricultural and Biological Sciences | 4 | 7% |
Medicine and Dentistry | 3 | 5% |
Immunology and Microbiology | 1 | 2% |
Other | 3 | 5% |
Unknown | 17 | 30% |