Chapter title |
Solubilization of proteins: the importance of lysis buffer choice.
|
---|---|
Chapter number | 8 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, Daniel J. MacPhee, Peach, Mandy, Marsh, Noelle, Miskiewicz, Ewa I., MacPhee, Daniel J. |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
The efficient extraction of proteins of interest from cells and tissues is not always straightforward. Here we demonstrate the differences in extraction of the focal adhesion protein Kindlin-2 from choriocarcinoma cells using NP-40 and RIPA lysis buffer. Furthermore, we demonstrate the use of a more denaturing urea/thiourea lysis buffer for solubilization, by comparing its effectiveness for solubilization of small heat-shock proteins from smooth muscle with the often utilized RIPA lysis buffer. Overall, the results demonstrate the importance of establishing the optimal lysis buffer for specific protein solubilization within the experimental workflow. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 134 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 33 | 25% |
Student > Ph. D. Student | 15 | 11% |
Researcher | 15 | 11% |
Student > Master | 8 | 6% |
Student > Doctoral Student | 6 | 4% |
Other | 14 | 10% |
Unknown | 43 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 30 | 22% |
Agricultural and Biological Sciences | 16 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 6% |
Medicine and Dentistry | 8 | 6% |
Neuroscience | 5 | 4% |
Other | 21 | 16% |
Unknown | 46 | 34% |