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Mendeley readers
Attention Score in Context
Chapter title |
Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
|
---|---|
Chapter number | 11 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Biji T. Kurien Ph.D., R. Hal Scofield M.D., Biji T. Kurien, R. Hal Scofield, Kurien, Biji T., Scofield, R. Hal |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
Western blotting enables the detection and characterization of proteins of low abundance. Sodium dodecyl sulfate (SDS) polyacrylamide gel-separated proteins are normally transferred electrophoretically to nitrocellulose or polyvinylidene difluoride membranes. Here we describe the transfer proteins [Ro 60 (or SSA) autoantigen, 220 and 240 kDa spectrin antigens, and prestained molecular weight standards] by diffusion from SDS polyacrylamide gels at 37 °C. Up to 12 immunoblots can be obtained from a single gel by this method. |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 27% |
Researcher | 11 | 23% |
Student > Master | 5 | 10% |
Student > Bachelor | 3 | 6% |
Other | 2 | 4% |
Other | 4 | 8% |
Unknown | 10 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 27% |
Biochemistry, Genetics and Molecular Biology | 11 | 23% |
Chemistry | 3 | 6% |
Immunology and Microbiology | 2 | 4% |
Neuroscience | 2 | 4% |
Other | 3 | 6% |
Unknown | 14 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2015.
All research outputs
#7,531,132
of 22,979,862 outputs
Outputs from Methods in molecular biology
#2,334
of 13,148 outputs
Outputs of similar age
#106,418
of 354,122 outputs
Outputs of similar age from Methods in molecular biology
#169
of 998 outputs
Altmetric has tracked 22,979,862 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,148 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 354,122 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 998 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.