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Mendeley readers
Chapter title |
B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells
|
---|---|
Chapter number | 42 |
Book title |
Western Blotting
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2694-7_42 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2693-0, 978-1-4939-2694-7
|
Authors |
Hemangi B. Shah Ph.D., Kristi A. Koelsch Ph.D., Hemangi B. Shah, Kristi A. Koelsch |
Editors |
Biji T. Kurien, R. Hal Scofield |
Abstract |
The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 4% |
Unknown | 26 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 30% |
Researcher | 5 | 19% |
Student > Ph. D. Student | 3 | 11% |
Student > Doctoral Student | 1 | 4% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 2 | 7% |
Unknown | 7 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 26% |
Immunology and Microbiology | 5 | 19% |
Medicine and Dentistry | 4 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Nursing and Health Professions | 1 | 4% |
Other | 1 | 4% |
Unknown | 7 | 26% |