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DNA Cloning and Assembly Methods

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Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

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    Book Overview
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    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
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    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
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    Chapter 3 Quick and Clean Cloning
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    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
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    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
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    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
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    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
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    Chapter 8 Circular polymerase extension cloning.
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    Chapter 9 Golden gate cloning.
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    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
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    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
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    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
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    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
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    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
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    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
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    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
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    Chapter 17 j5 DNA Assembly Design Automation
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    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 13: Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
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