↓ Skip to main content

DNA Cloning and Assembly Methods

Overview of attention for book
Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
  3. Altmetric Badge
    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
  4. Altmetric Badge
    Chapter 3 Quick and Clean Cloning
  5. Altmetric Badge
    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
  6. Altmetric Badge
    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
  7. Altmetric Badge
    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
  8. Altmetric Badge
    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
  9. Altmetric Badge
    Chapter 8 Circular polymerase extension cloning.
  10. Altmetric Badge
    Chapter 9 Golden gate cloning.
  11. Altmetric Badge
    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
  12. Altmetric Badge
    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
  13. Altmetric Badge
    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
  14. Altmetric Badge
    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
  15. Altmetric Badge
    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
  16. Altmetric Badge
    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
  17. Altmetric Badge
    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
  18. Altmetric Badge
    Chapter 17 j5 DNA Assembly Design Automation
  19. Altmetric Badge
    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 13: Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
Altmetric Badge

Mentioned by

facebook
1 Facebook page

Citations

dimensions_citation
12 Dimensions

Readers on

mendeley
29 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
Chapter number 13
Book title
DNA Cloning and Assembly Methods
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-764-8_13
Pubmed ID
Book ISBNs
978-1-62703-763-1, 978-1-62703-764-8
Authors

Chun You, Y.-H. Percival Zhang

Abstract

We developed a simple method (Simple Cloning) for subcloning one, two, or three DNA fragments into any location of a targeted vector without the need for restriction enzyme, ligase, exonuclease, or recombinase. This cloning technology can be applied to a few common Escherichia coli hosts (e.g., BL21(DE3), DH5α, JM109, TOP10). The protocol includes three steps: (a) linear DNA fragments (i.e., the insert DNA and the vector backbone) with two overlap ends were generated by regular high-fidelity PCR, (b) the DNA multimers were generated based on these equimolar DNA templates by using prolonged overlap extension PCR (POE-PCR) without primers added, and (c) the POE-PCR product was transformed to E. coli strains directly. Because positive colony efficiencies are very high, it is not necessary to identify desired clones by using colony PCR. Simple Cloning provides a new cloning and DNA assembly method with great simplicity and flexibility.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 34%
Researcher 8 28%
Lecturer 2 7%
Professor 1 3%
Student > Master 1 3%
Other 1 3%
Unknown 6 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 45%
Biochemistry, Genetics and Molecular Biology 4 14%
Materials Science 1 3%
Unknown 11 38%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 July 2014.
All research outputs
#15,349,970
of 17,368,632 outputs
Outputs from Methods in molecular biology
#6,845
of 9,919 outputs
Outputs of similar age
#161,827
of 195,064 outputs
Outputs of similar age from Methods in molecular biology
#24
of 80 outputs
Altmetric has tracked 17,368,632 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,919 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 195,064 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 80 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.