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DNA Cloning and Assembly Methods

Overview of attention for book
Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
  3. Altmetric Badge
    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
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    Chapter 3 Quick and Clean Cloning
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    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
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    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
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    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
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    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
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    Chapter 8 Circular polymerase extension cloning.
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    Chapter 9 Golden gate cloning.
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    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
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    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
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    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
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    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
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    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
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    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
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    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
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    Chapter 17 j5 DNA Assembly Design Automation
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    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 1: BioBrick Assembly Standards and Techniques and Associated Software Tools.
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  • High Attention Score compared to outputs of the same age and source (86th percentile)

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Chapter title
BioBrick Assembly Standards and Techniques and Associated Software Tools.
Chapter number 1
Book title
DNA Cloning and Assembly Methods
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-764-8_1
Pubmed ID
Book ISBNs
978-1-62703-763-1, 978-1-62703-764-8
Authors

Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås, Rahmi Lale, Røkke G, Korvald E, Pahr J, Oyås O, Lale R, Røkke, Gunvor, Korvald, Eirin, Pahr, Jarle, Øyås, Ove, Lale, Rahmi

Abstract

The BioBrick idea was developed to introduce the engineering principles of abstraction and standardization into synthetic biology. BioBricks are DNA sequences that serve a defined biological function and can be readily assembled with any other BioBrick parts to create new BioBricks with novel properties. In order to achieve this, several assembly standards can be used. Which assembly standards a BioBrick is compatible with, depends on the prefix and suffix sequences surrounding the part. In this chapter, five of the most common assembly standards will be described, as well as some of the most used assembly techniques, cloning procedures, and a presentation of the available software tools that can be used for deciding on the best method for assembling of different BioBricks, and searching for BioBrick parts in the Registry of Standard Biological Parts database.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 1%
Ireland 1 1%
Unknown 88 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 17 19%
Student > Ph. D. Student 11 12%
Researcher 10 11%
Student > Master 10 11%
Professor 4 4%
Other 11 12%
Unknown 27 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 32 36%
Agricultural and Biological Sciences 11 12%
Chemical Engineering 4 4%
Engineering 3 3%
Nursing and Health Professions 2 2%
Other 5 6%
Unknown 33 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2021.
All research outputs
#6,129,783
of 22,739,983 outputs
Outputs from Methods in molecular biology
#1,812
of 13,087 outputs
Outputs of similar age
#72,223
of 305,211 outputs
Outputs of similar age from Methods in molecular biology
#80
of 594 outputs
Altmetric has tracked 22,739,983 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 13,087 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,211 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.