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DNA Cloning and Assembly Methods

Overview of attention for book
Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

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    Book Overview
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    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
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    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
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    Chapter 3 Quick and Clean Cloning
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    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
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    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
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    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
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    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
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    Chapter 8 Circular polymerase extension cloning.
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    Chapter 9 Golden gate cloning.
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    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
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    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
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    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
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    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
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    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
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    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
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    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
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    Chapter 17 j5 DNA Assembly Design Automation
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    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 11: FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
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Citations

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Chapter title
FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
Chapter number 11
Book title
DNA Cloning and Assembly Methods
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-764-8_11
Pubmed ID
Book ISBNs
978-1-62703-763-1, 978-1-62703-764-8
Authors

Eric R. Geertsma

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 26%
Student > Ph. D. Student 5 16%
Student > Postgraduate 3 10%
Student > Master 3 10%
Professor > Associate Professor 2 6%
Other 4 13%
Unknown 6 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 39%
Biochemistry, Genetics and Molecular Biology 10 32%
Immunology and Microbiology 1 3%
Medicine and Dentistry 1 3%
Unknown 7 23%