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DNA Cloning and Assembly Methods

Overview of attention for book
Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
  3. Altmetric Badge
    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
  4. Altmetric Badge
    Chapter 3 Quick and Clean Cloning
  5. Altmetric Badge
    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
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    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
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    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
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    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
  9. Altmetric Badge
    Chapter 8 Circular polymerase extension cloning.
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    Chapter 9 Golden gate cloning.
  11. Altmetric Badge
    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
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    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
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    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
  14. Altmetric Badge
    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
  15. Altmetric Badge
    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
  16. Altmetric Badge
    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
  17. Altmetric Badge
    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
  18. Altmetric Badge
    Chapter 17 j5 DNA Assembly Design Automation
  19. Altmetric Badge
    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 17: j5 DNA Assembly Design Automation
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (74th percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

Mentioned by

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7 tweeters

Citations

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12 Dimensions

Readers on

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110 Mendeley
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Chapter title
j5 DNA Assembly Design Automation
Chapter number 17
Book title
DNA Cloning and Assembly Methods
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-764-8_17
Pubmed ID
Book ISBNs
978-1-62703-763-1, 978-1-62703-764-8
Authors

Nathan J. Hillson

Abstract

Modern standardized methodologies, described in detail in the previous chapters of this book, have enabled the software-automated design of optimized DNA construction protocols. This chapter describes how to design (combinatorial) scar-less DNA assembly protocols using the web-based software j5. j5 assists biomedical and biotechnological researchers construct DNA by automating the design of optimized protocols for flanking homology sequence as well as type IIS endonuclease-mediated DNA assembly methodologies. Unlike any other software tool available today, j5 designs scar-less combinatorial DNA assembly protocols, performs a cost-benefit analysis to identify which portions of an assembly process would be less expensive to outsource to a DNA synthesis service provider, and designs hierarchical DNA assembly strategies to mitigate anticipated poor assembly junction sequence performance. Software integrated with j5 add significant value to the j5 design process through graphical user-interface enhancement and downstream liquid-handling robotic laboratory automation.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 110 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 6 5%
France 1 <1%
Sweden 1 <1%
Switzerland 1 <1%
United Kingdom 1 <1%
South Africa 1 <1%
Japan 1 <1%
Belgium 1 <1%
Unknown 97 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 26%
Researcher 25 23%
Student > Master 12 11%
Student > Bachelor 7 6%
Student > Doctoral Student 6 5%
Other 16 15%
Unknown 15 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 47 43%
Biochemistry, Genetics and Molecular Biology 28 25%
Medicine and Dentistry 3 3%
Computer Science 3 3%
Immunology and Microbiology 2 2%
Other 8 7%
Unknown 19 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2014.
All research outputs
#5,858,314
of 21,192,559 outputs
Outputs from Methods in molecular biology
#1,677
of 11,944 outputs
Outputs of similar age
#76,642
of 303,263 outputs
Outputs of similar age from Methods in molecular biology
#81
of 667 outputs
Altmetric has tracked 21,192,559 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 11,944 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 303,263 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 667 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.