↓ Skip to main content

DNA Cloning and Assembly Methods

Overview of attention for book
Cover of 'DNA Cloning and Assembly Methods'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 BioBrick Assembly Standards and Techniques and Associated Software Tools.
  3. Altmetric Badge
    Chapter 2 Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
  4. Altmetric Badge
    Chapter 3 Quick and Clean Cloning
  5. Altmetric Badge
    Chapter 4 Hierarchical ligation-independent assembly of PCR fragments.
  6. Altmetric Badge
    Chapter 5 USER-Derived Cloning Methods and Their Primer Design
  7. Altmetric Badge
    Chapter 6 Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
  8. Altmetric Badge
    Chapter 7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
  9. Altmetric Badge
    Chapter 8 Circular polymerase extension cloning.
  10. Altmetric Badge
    Chapter 9 Golden gate cloning.
  11. Altmetric Badge
    Chapter 10 Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
  12. Altmetric Badge
    Chapter 11 FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
  13. Altmetric Badge
    Chapter 12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
  14. Altmetric Badge
    Chapter 13 Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
  15. Altmetric Badge
    Chapter 14 Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
  16. Altmetric Badge
    Chapter 15 Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
  17. Altmetric Badge
    Chapter 16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method.
  18. Altmetric Badge
    Chapter 17 j5 DNA Assembly Design Automation
  19. Altmetric Badge
    Chapter 18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis.
Attention for Chapter 7: A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (76th percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

Mentioned by

blogs
1 blog

Citations

dimensions_citation
12 Dimensions

Readers on

mendeley
33 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform.
Chapter number 7
Book title
DNA Cloning and Assembly Methods
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-764-8_7
Pubmed ID
Book ISBNs
978-1-62703-763-1, 978-1-62703-764-8
Authors

Erijman A, Shifman JM, Peleg Y, Ariel Erijman, Julia M. Shifman, Yoav Peleg

Abstract

DNA cloning is a basic methodology employed for multiple applications in all life-science disciplines. In order to facilitate DNA cloning we developed Transfer-PCR (TPCR), a novel approach that integrates in a single tube, PCR amplification of the target DNA from an origin vector and its subsequent integration into the destination vector. TPCR can be applied for incorporation of DNA fragments into any desired position within a circular plasmid without the need for purification of the intermediate PCR product and without the use of any commercial kit. TPCR reaction is most efficient within a narrow range of primer concentrations. Adaptation of the TPCR should facilitate, simplify, and significantly reduce time and costs for DNA assembly, as well as protein engineering studies. In the current publication we describe a detailed protocol for implementation of the TPCR method for DNA assembly.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 3%
Unknown 32 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 39%
Student > Bachelor 3 9%
Student > Ph. D. Student 3 9%
Student > Doctoral Student 2 6%
Professor > Associate Professor 2 6%
Other 6 18%
Unknown 4 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 39%
Agricultural and Biological Sciences 9 27%
Chemistry 2 6%
Environmental Science 1 3%
Neuroscience 1 3%
Other 1 3%
Unknown 6 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2015.
All research outputs
#4,469,573
of 17,365,229 outputs
Outputs from Methods in molecular biology
#1,192
of 9,916 outputs
Outputs of similar age
#71,212
of 311,850 outputs
Outputs of similar age from Methods in molecular biology
#105
of 877 outputs
Altmetric has tracked 17,365,229 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 9,916 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,850 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 877 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.