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Microbial Proteomics

Overview of attention for book
Cover of 'Microbial Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 Filter-Aided Sample Preparation for Proteome Analysis
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    Chapter 2 Protein Enrichment from Highly Dilute Samples with StrataClean
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    Chapter 3 Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins
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    Chapter 4 Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis
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    Chapter 5 Preparation of Bacterial Magnetosomes for Proteome Analysis
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    Chapter 6 Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics
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    Chapter 7 Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS
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    Chapter 8 Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms
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    Chapter 9 Proteomic Signatures in Staphylococcus aureus
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    Chapter 10 How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis
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    Chapter 11 Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE
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    Chapter 12 Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses
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    Chapter 13 Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)
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    Chapter 14 Next-Generation Trapping of Protease Substrates by Label-Free Proteomics
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    Chapter 15 In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings
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    Chapter 16 Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography
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    Chapter 17 Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation
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    Chapter 18 Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria
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    Chapter 19 Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host–Microbiota Interactions
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    Chapter 20 Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis/Proteome Extraction from Soil Samples for Metaproteomics Studies
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    Chapter 21 Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter
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    Chapter 22 Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria–Invertebrate Symbioses
Attention for Chapter 15: In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings
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Chapter title
In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings
Chapter number 15
Book title
Microbial Proteomics
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8695-8_15
Pubmed ID
Book ISBNs
978-1-4939-8693-4, 978-1-4939-8695-8
Authors

Henrike Pförtner, Maren Depke, Kristin Surmann, Frank Schmidt, Uwe Völker, Pförtner, Henrike, Depke, Maren, Surmann, Kristin, Schmidt, Frank, Völker, Uwe

Abstract

Proteome profiling of bacteria internalized by host cells is still a challenging task, due to low amounts of bacterial proteins in host-pathogen settings and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 106 internalized bacterial cells as starting material.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Student > Bachelor 1 100%
Researcher 1 100%
Readers by discipline Count As %
Environmental Science 1 100%
Immunology and Microbiology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2018.
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#20,535,139
of 23,105,443 outputs
Outputs from Methods in molecular biology
#9,985
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Outputs of similar age
#297,074
of 341,808 outputs
Outputs of similar age from Methods in molecular biology
#170
of 225 outputs
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