Chapter title |
Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter
|
---|---|
Chapter number | 21 |
Book title |
Microbial Proteomics
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8695-8_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8693-4, 978-1-4939-8695-8
|
Authors |
Katharina M. Keiblinger, Katharina Riedel, Keiblinger, Katharina M., Riedel, Katharina |
Abstract |
Soil and litter metaproteomics, assigning soil and litter proteins to specific phylogenetic and functional groups, has a great potential to shed light on the impact of microbial diversity on soil ecosystem functioning. However, metaproteomic analysis of soil and litter is often hampered by the enormous heterogeneity of the soil matrix and high concentrations of humic acids. To circumvent these challenges, sophisticated protocols for sample preparation have to be applied. This chapter provides the reader with detailed information on well-established protocols for protein extraction from soil and litter samples together with protocols for further sample preparation for subsequent MS analyses. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 36% |
Professor > Associate Professor | 2 | 14% |
Other | 1 | 7% |
Student > Master | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Other | 2 | 14% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 29% |
Environmental Science | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
Unknown | 5 | 36% |