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Mendeley readers
| Chapter title |
Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)
|
|---|---|
| Chapter number | 13 |
| Book title |
Microbial Proteomics
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8695-8_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8693-4, 978-1-4939-8695-8
|
| Authors |
Andreas Otto, Otto, Andreas |
| Abstract |
Quantitative Proteomics based on stable isotope labeling by 15N is a versatile method in determination of changes in microbial protein expression profiles. Once set up, the workflow relies on defined chemical or commercially available complex media leading to universal heavy labeling by the stable isotope 15N. This chapter provides a detailed protocol for labeling of different bacteria and the strategy for evaluating the incorporation rate of the heavy stable isotopes in the sample is discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 27% |
| Librarian | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Unspecified | 1 | 9% |
| Professor | 1 | 9% |
| Other | 3 | 27% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 36% |
| Agricultural and Biological Sciences | 2 | 18% |
| Environmental Science | 1 | 9% |
| Unspecified | 1 | 9% |
| Immunology and Microbiology | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |