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Microbial Proteomics

Overview of attention for book
Cover of 'Microbial Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 Filter-Aided Sample Preparation for Proteome Analysis
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    Chapter 2 Protein Enrichment from Highly Dilute Samples with StrataClean
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    Chapter 3 Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins
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    Chapter 4 Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis
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    Chapter 5 Preparation of Bacterial Magnetosomes for Proteome Analysis
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    Chapter 6 Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics
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    Chapter 7 Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS
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    Chapter 8 Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms
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    Chapter 9 Proteomic Signatures in Staphylococcus aureus
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    Chapter 10 How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis
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    Chapter 11 Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE
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    Chapter 12 Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses
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    Chapter 13 Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)
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    Chapter 14 Next-Generation Trapping of Protease Substrates by Label-Free Proteomics
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    Chapter 15 In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings
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    Chapter 16 Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography
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    Chapter 17 Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation
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    Chapter 18 Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria
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    Chapter 19 Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host–Microbiota Interactions
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    Chapter 20 Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis/Proteome Extraction from Soil Samples for Metaproteomics Studies
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    Chapter 21 Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter
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    Chapter 22 Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria–Invertebrate Symbioses
Attention for Chapter 6: Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics
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Chapter title
Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics
Chapter number 6
Book title
Microbial Proteomics
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8695-8_6
Pubmed ID
30259480
Book ISBNs
978-1-4939-8693-4, 978-1-4939-8695-8
Authors

Christian Manske, Ivo Finsel, Christine Hoffmann, Hubert Hilbi, Manske, Christian, Finsel, Ivo, Hoffmann, Christine, Hilbi, Hubert

Abstract

The causative agent of Legionnaires' disease, Legionella pneumophila, replicates in free-living amoebae as well as in macrophages of the innate immune system within a distinct membrane-bound compartment, the "Legionella-containing-vacuole" (LCV). LCV formation is a complex process and requires the bacterial Icm/Dot type IV secretion system, which translocates approximately 300 different "effector" proteins. Intact LCVs from infected Dictyostelium discoideum amoebae or RAW 264.7 murine macrophages can be purified using a straightforward protocol. In the first step, the LCVs in cell homogenates are tagged with an antibody directed against an L. pneumophila effector protein specifically localizing to the pathogen vacuole membrane and isolated by immunomagnetic separation using a secondary antibody coupled to magnetic beads. In the second step, the LCVs are further enriched by density gradient centrifugation through a Histodenz cushion. LCVs thus purified are analyzed by mass spectrometry-based proteomics and characterized by biochemical and cell biological approaches.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 17%
Student > Doctoral Student 1 17%
Student > Bachelor 1 17%
Student > Ph. D. Student 1 17%
Student > Master 1 17%
Other 0 0%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 33%
Chemistry 1 17%
Medicine and Dentistry 1 17%
Unknown 2 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2018.
All research outputs
#18,650,639
of 23,105,443 outputs
Outputs from Methods in molecular biology
#7,996
of 13,223 outputs
Outputs of similar age
#261,615
of 341,808 outputs
Outputs of similar age from Methods in molecular biology
#146
of 225 outputs
Altmetric has tracked 23,105,443 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,223 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 225 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.

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