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Chapter title |
Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses
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Chapter number | 12 |
Book title |
Microbial Proteomics
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Published in |
Methods in molecular biology, September 2018
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DOI | 10.1007/978-1-4939-8695-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8693-4, 978-1-4939-8695-8
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Authors |
Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, Frank Schmidt, Herbst, Florian-Alexander, Jehmlich, Nico, Bergen, Martin von, Schmidt, Frank |
Abstract |
Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 33% |
Unspecified | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 17% |
Environmental Science | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Chemistry | 1 | 17% |
Unknown | 2 | 33% |