Chapter title |
Preparation of Bacterial Magnetosomes for Proteome Analysis
|
---|---|
Chapter number | 5 |
Book title |
Microbial Proteomics
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8695-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8693-4, 978-1-4939-8695-8
|
Authors |
Oliver Raschdorf, Dirk Schüler, René Uebe, Raschdorf, Oliver, Schüler, Dirk, Uebe, René |
Abstract |
Magnetotactic bacteria form unique prokaryotic organelles, termed magnetosomes, which consist of membrane-enclosed magnetite nanoparticles. Analysis of magnetosome biogenesis has been greatly facilitated by proteomic methods. These, however, require pure, highly enriched magnetosomes. Here, we describe the purification of magnetosomes from Magnetospirillum gryphiswaldense using high pressure cell disruption, and sequential purification by magnetic enrichment and sucrose density ultracentrifugation. The resulting enriched magnetosomes can be subsequently subjected to proteomic analyses or biotechnological applications. |
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