Chapter title |
Evaluation of Autoreactive Responses
|
---|---|
Chapter number | 21 |
Book title |
Rheumatoid Arthritis
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8802-0_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8801-3, 978-1-4939-8802-0
|
Authors |
Shuang Liu, Liu, Shuang |
Abstract |
Loss of tolerance to self-antigens is considered to be one of the initial reasons for the onset of rheumatoid arthritis (RA). Identification of self-antigens and evaluation of autoreactive antibodies can foster understanding of the pathogenesis of the disease and the development of new therapeutics. By detection of responses to a particular self-antigen, such as α-enolase, keratin, fibrinogen, fibronectin, collagen, or vimentin, in patient- and animal model-derived samples, high-affinity T-cell receptor-dependent activation of autoreactive T cells to self-antigens can be elucidated. This chapter introduces a simple method to estimate T-cell autoreactive responses to CII in a murine CIA model. A limiting dilution system is established in order to assess CII-dependent T-cell responses, which are reflected by the level of cytokine release. |
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