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Mendeley readers
Chapter title |
Lentiviral-Mediated Systemic RNA Interference In Vivo
|
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Chapter number | 14 |
Book title |
Rheumatoid Arthritis
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Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8802-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8801-3, 978-1-4939-8802-0
|
Authors |
Shuang Liu |
Abstract |
The shRNA-encoding lentivirus has been widely used for gene manipulation in preclinical studies. It is a powerful tool for gene transfer and shows promise in its ability to efficiently transduce immune cells and hematopoietic stem cells, which are the initial therapeutic target of autoimmune diseases, considering that gene manipulation of these cells is usually difficult to achieve using other techniques. In previous chapters, we have described how to produce concentrated shRNA-encoding lentiviral particles. Here, systemic in vivo application of lentivirus, including viral quantification prior to injection, intraperitoneal injection, and quantification of integrated provirus, is introduced. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 50% |
Unknown | 1 | 50% |