Chapter title |
Generation of Specific Aptamers
|
---|---|
Chapter number | 11 |
Book title |
Rheumatoid Arthritis
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8802-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8801-3, 978-1-4939-8802-0
|
Authors |
Shuang Liu, Yasuyuki Suzuki, Makoto Inui |
Abstract |
Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained. |
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Professor | 1 | 25% |
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