| Chapter title |
Screening of Ca2+ Influx in Lymphocytes
|
|---|---|
| Chapter number | 16 |
| Book title |
Rheumatoid Arthritis
|
| Published in |
Methods in molecular biology, September 2018
|
| DOI | 10.1007/978-1-4939-8802-0_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8801-3, 978-1-4939-8802-0
|
| Authors |
Erika Takemasa, Shuang Liu |
| Abstract |
The Ca2+ ion is an important second messenger in lymphocytes, similarly to its function in other mammalian cells. The generation of long-lasting intracellular Ca2+ elevations is essential for Ca2+-dependent gene transcription, proliferation, differentiation, and cytokine production in lymphocytes. Since store-operated Ca2+ entry (SOCE) is considered the predominant mode of Ca2+ influx in lymphocytes, the activation and function of lymphocytes can be generally predicted by monitoring SOCE. A method suitable for dynamic monitoring of Ca2+ influx using fura-2 labeling in lymphocytes is introduced in this chapter. Using this technique, large-scale screening of the activation status of primary or cultured lymphocytes can be realized. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 50% |
| Student > Postgraduate | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Nursing and Health Professions | 1 | 50% |
| Unknown | 1 | 50% |