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The Genetic Manipulation of Staphylococci

Overview of attention for book
Cover of 'The Genetic Manipulation of Staphylococci'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 180 Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus.
  3. Altmetric Badge
    Chapter 181 The Genetic Manipulation of Staphylococci
  4. Altmetric Badge
    Chapter 182 Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
  5. Altmetric Badge
    Chapter 183 Method for Preparation and Electroporation of S. aureus and S. epidermidis
  6. Altmetric Badge
    Chapter 184 Rapid Isolation of DNA from Staphylococcus
  7. Altmetric Badge
    Chapter 185 Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.
  8. Altmetric Badge
    Chapter 186 Bacteriophage Transduction in Staphylococcus aureus.
  9. Altmetric Badge
    Chapter 187 Allelic Exchange.
  10. Altmetric Badge
    Chapter 188 Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.
  11. Altmetric Badge
    Chapter 189 Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis
  12. Altmetric Badge
    Chapter 190 Chemical and UV Mutagenesis.
  13. Altmetric Badge
    Chapter 191 Pulse Field Gel Electrophoresis.
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    Chapter 192 RNA-Sequencing of Staphylococcus aureus Messenger RNA.
  15. Altmetric Badge
    Chapter 193 Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression.
  16. Altmetric Badge
    Chapter 275 De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.
  17. Altmetric Badge
    Chapter 276 The Genetic Manipulation of Staphylococci
  18. Altmetric Badge
    Chapter 277 Electrophoretic Mobility Shift Assays
  19. Altmetric Badge
    Chapter 281 Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
  20. Altmetric Badge
    Chapter 282 Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus
  21. Altmetric Badge
    Chapter 283 The Genetic Manipulation of Staphylococci
Attention for Chapter 275: De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

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Chapter title
De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.
Chapter number 275
Book title
The Genetic Manipulation of Staphylococci
Published in
Methods in molecular biology, July 2015
DOI 10.1007/7651_2015_275
Pubmed ID
Book ISBNs
978-1-4939-3157-6, 978-1-4939-3158-3
Authors

Mashruwala, Ameya A, Boyd, Jeffrey M, Ameya A. Mashruwala, Jeffrey M. Boyd, Mashruwala, Ameya A., Boyd, Jeffrey M.

Abstract

Molecular cloning is a cornerstone of modern biology laboratories. However, traditional cloning can be time-consuming and problematic. We outline herein a method that utilizes the endogenous gap repair system of yeast cells to clone and assemble DNA constructs. This system is simple, cheap, and requires minimal reagents. It can be used for the assembly of both simple (single DNA fragments) and complex (multiple DNA fragments) constructs into plasmids.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 50%
Student > Bachelor 2 20%
Professor 1 10%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 60%
Agricultural and Biological Sciences 2 20%
Unknown 2 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2022.
All research outputs
#3,858,701
of 23,408,972 outputs
Outputs from Methods in molecular biology
#970
of 13,324 outputs
Outputs of similar age
#48,820
of 265,367 outputs
Outputs of similar age from Methods in molecular biology
#1
of 20 outputs
Altmetric has tracked 23,408,972 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,367 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.