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Mendeley readers
| Chapter title |
Electrophoretic Mobility Shift Assays
|
|---|---|
| Chapter number | 277 |
| Book title |
The Genetic Manipulation of Staphylococci
|
| Published in |
Methods in molecular biology, July 2015
|
| DOI | 10.1007/7651_2015_277 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3157-6, 978-1-4939-3158-3
|
| Authors |
Sarah E. Rowe, James P. O’Gara, Rowe, Sarah E., O’Gara, James P. |
| Abstract |
Experimental demonstration of regulatory protein interactions with the sequences upstream of potential target genes is an important element in gene expression studies. These experiments termed electrophoretic mobility shift assays (EMSAs) provide valuable insight into the mechanism of action of transcription factors. EMSAs combined with downstream applications such as transcriptional analysis help uncover precisely how regulatory proteins control target gene expression. This chapter comprises a guideline for expression and purification of recombinant transcription factor proteins followed by a detailed protocol for EMSAs. |
Mendeley readers
The data shown below were compiled from readership statistics for 241 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| India | 3 | 1% |
| United Kingdom | 2 | <1% |
| Bolivia, Plurinational State of | 1 | <1% |
| South Africa | 1 | <1% |
| Chile | 1 | <1% |
| Switzerland | 1 | <1% |
| Spain | 1 | <1% |
| Canada | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 225 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 75 | 31% |
| Student > Bachelor | 36 | 15% |
| Researcher | 33 | 14% |
| Student > Master | 26 | 11% |
| Student > Doctoral Student | 10 | 4% |
| Other | 35 | 15% |
| Unknown | 26 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 110 | 46% |
| Biochemistry, Genetics and Molecular Biology | 59 | 24% |
| Medicine and Dentistry | 13 | 5% |
| Chemistry | 10 | 4% |
| Immunology and Microbiology | 6 | 2% |
| Other | 11 | 5% |
| Unknown | 32 | 13% |