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Mendeley readers
| Chapter title |
Method for Preparation and Electroporation of S. aureus and S. epidermidis
|
|---|---|
| Chapter number | 183 |
| Book title |
The Genetic Manipulation of Staphylococci
|
| Published in |
Methods in molecular biology, May 2015
|
| DOI | 10.1007/7651_2014_183 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3157-6, 978-1-4939-3158-3
|
| Authors |
Melinda R. Grosser, Anthony R. Richardson, Grosser, Melinda R., Richardson, Anthony R. |
| Abstract |
For bacterial species that are not known to be naturally competent, such as Staphylococcus aureus and Staphylococcus epidermidis, electroporation is an efficient method for introducing genetic material into the cell. The technique utilizes electrical pulses to transiently permeabilize bacterial cell membranes, which allows for the passage of plasmid DNA across the membranes. Here, we describe methods for preparing electrocompetent S. aureus and S. epidermidis cells and outline a procedure for electroporation of the prepared competent cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 68 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 13 | 19% |
| Student > Master | 9 | 13% |
| Student > Ph. D. Student | 8 | 12% |
| Researcher | 6 | 9% |
| Student > Postgraduate | 6 | 9% |
| Other | 10 | 15% |
| Unknown | 16 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 26% |
| Immunology and Microbiology | 12 | 18% |
| Agricultural and Biological Sciences | 11 | 16% |
| Chemistry | 3 | 4% |
| Unspecified | 2 | 3% |
| Other | 2 | 3% |
| Unknown | 20 | 29% |