Chapter title |
Chemical and UV Mutagenesis.
|
---|---|
Chapter number | 190 |
Book title |
The Genetic Manipulation of Staphylococci
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/7651_2014_190 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3157-6, 978-1-4939-3158-3
|
Authors |
Jeffrey L Bose, Bose JL, Jeffrey L. Bose, Bose, Jeffrey L. |
Abstract |
The ability to create mutations is an important step towards understanding bacterial physiology and virulence. While targeted approaches are invaluable, the ability to produce genome-wide random mutations can lead to crucial discoveries. Transposon mutagenesis is a useful approach, but many interesting mutations can be missed by these insertions that interrupt coding and noncoding sequences due to the integration of an entire transposon. Chemical mutagenesis and UV-based random mutagenesis are alternate approaches to isolate mutations of interest with the potential of only single nucleotide changes. Once a standard method, difficulty in identifying mutation sites had decreased the popularity of this technique. However, thanks to the recent emergence of economical whole-genome sequencing, this approach to making mutations can once again become a viable option. Therefore, this chapter provides an overview protocol for random mutagenesis using UV light or DNA-damaging chemicals. |
X Demographics
Geographical breakdown
Country | Count | As % |
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India | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Mexico | 1 | 1% |
Unknown | 84 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 25% |
Student > Bachelor | 17 | 20% |
Student > Master | 13 | 15% |
Researcher | 6 | 7% |
Student > Doctoral Student | 4 | 5% |
Other | 5 | 6% |
Unknown | 19 | 22% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 25 | 29% |
Agricultural and Biological Sciences | 19 | 22% |
Immunology and Microbiology | 7 | 8% |
Engineering | 4 | 5% |
Chemical Engineering | 3 | 4% |
Other | 7 | 8% |
Unknown | 20 | 24% |