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Mendeley readers
| Chapter title |
Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
|
|---|---|
| Chapter number | 281 |
| Book title |
The Genetic Manipulation of Staphylococci
|
| Published in |
Methods in molecular biology, July 2015
|
| DOI | 10.1007/7651_2015_281 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3157-6, 978-1-4939-3158-3
|
| Authors |
Katherine L. Maliszewski, Maliszewski, Katherine L. |
| Abstract |
In the gram-positive pathogen Staphylococcus aureus, transposon mutagenesis is a useful method of screening large numbers of mutants for a given phenotype. However, constructing a transposon mutant library can take several months of work and validation in the laboratory. In this chapter, we describe a method for batch transduction of existing transposon mutations into new genetic backgrounds. Transduction in S. aureus is accomplished quickly and easily in most commonly used laboratory strains. The method described herein utilizes transduction to facilitate the rapid creation of new libraries and quick screening in strains containing phenotypic reporter constructs. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 50% |
| Researcher | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 1 | 50% |
| Agricultural and Biological Sciences | 1 | 50% |