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The Genetic Manipulation of Staphylococci

Overview of attention for book
Cover of 'The Genetic Manipulation of Staphylococci'

Table of Contents

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    Book Overview
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    Chapter 180 Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus.
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    Chapter 181 The Genetic Manipulation of Staphylococci
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    Chapter 182 Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products
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    Chapter 183 Method for Preparation and Electroporation of S. aureus and S. epidermidis
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    Chapter 184 Rapid Isolation of DNA from Staphylococcus
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    Chapter 185 Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.
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    Chapter 186 Bacteriophage Transduction in Staphylococcus aureus.
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    Chapter 187 Allelic Exchange.
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    Chapter 188 Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.
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    Chapter 189 Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using bursa aurealis
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    Chapter 190 Chemical and UV Mutagenesis.
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    Chapter 191 Pulse Field Gel Electrophoresis.
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    Chapter 192 RNA-Sequencing of Staphylococcus aureus Messenger RNA.
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    Chapter 193 Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression.
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    Chapter 275 De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.
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    Chapter 276 The Genetic Manipulation of Staphylococci
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    Chapter 277 Electrophoretic Mobility Shift Assays
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    Chapter 281 Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus
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    Chapter 282 Rapid Amplification of cDNA Ends for RNA Transcript Sequencing in Staphylococcus
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    Chapter 283 The Genetic Manipulation of Staphylococci
Attention for Chapter 193: Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression.
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Chapter title
Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression.
Chapter number 193
Book title
The Genetic Manipulation of Staphylococci
Published in
Methods in molecular biology, February 2015
DOI 10.1007/7651_2014_193
Pubmed ID
25646613
Book ISBNs
978-1-4939-3157-6, 978-1-4939-3158-3
Authors

April M Lewis, Kelly C Rice, April M. Lewis, Kelly C. Rice, Lewis, April M., Rice, Kelly C.

Abstract

Quantitative real-time polymerase chain reaction (qPCR) is a sensitive tool that can be used to quantify and compare the amount of specific RNA transcripts between different biological samples. This chapter describes the use of a "two-step" qPCR method to calculate the relative fold change of expression of genes of interest in S. aureus. Using this work-flow, cDNA is synthesized from RNA templates (previously checked for the absence of significant genomic DNA contamination) using a cocktail of random primers and reverse-transcriptase enzyme. The cDNA pools generated can then be assessed for expression of specific genes of interest using SYBR Green-based qPCR and quantification of relative fold-change expression.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 12%
Researcher 2 12%
Student > Master 2 12%
Lecturer 1 6%
Other 1 6%
Other 3 18%
Unknown 6 35%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 29%
Agricultural and Biological Sciences 2 12%
Immunology and Microbiology 2 12%
Medicine and Dentistry 1 6%
Engineering 1 6%
Other 0 0%
Unknown 6 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2015.
All research outputs
#18,395,201
of 22,785,242 outputs
Outputs from Methods in molecular biology
#7,890
of 13,094 outputs
Outputs of similar age
#256,622
of 352,352 outputs
Outputs of similar age from Methods in molecular biology
#458
of 977 outputs
Altmetric has tracked 22,785,242 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,094 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,352 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 977 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.

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