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Molecular Typing of Blood Cell Antigens

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Cover of 'Molecular Typing of Blood Cell Antigens'

Table of Contents

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    Book Overview
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    Chapter 1 Validation of Genotyping Protocols for Diagnostic Use
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    Chapter 2 High-resolution melting analysis of single nucleotide polymorphisms.
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    Chapter 3 High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping of Single Nucleotide Polymorphisms
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    Chapter 4 Blood Grouping Based on PCR Methods and Agarose Gel Electrophoresis
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    Chapter 5 Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry
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    Chapter 6 PCR with Sequence-Specific Primers for Typing of Diallelic Blood Groups
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    Chapter 7 High-resolution melting analysis for genotyping duffy blood group antigens.
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    Chapter 8 Molecular RHD - RHCE Analysis by Multiplex PCR of Short Fluorescent Fragments
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    Chapter 9 Microarrays in Blood Group Genotyping
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    Chapter 10 Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status.
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    Chapter 11 454-Sequencing™ for the KEL, JR, and LAN Blood Groups.
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    Chapter 12 Noninvasive Prenatal Blood Group Genotyping
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    Chapter 13 Genotyping of Human Platelet Antigens by BeadChip Microarray Technology
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    Chapter 14 Sequence-Based Typing for Platelet alloantigens
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    Chapter 15 Miniaturized Technology for DNA Typing: Cassette PCR
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    Chapter 16 Geno- and Phenotyping of Human Neutrophil Antigens
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    Chapter 17 Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
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    Chapter 18 Novel Approaches and Technologies in Molecular HLA Typing.
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    Chapter 19 Luminex-Based Methods in High-Resolution HLA Typing
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    Chapter 20 In Silico HLA Typing Using Standard RNA-Seq Sequence Reads.
Attention for Chapter 11: 454-Sequencing™ for the KEL, JR, and LAN Blood Groups.
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Chapter title
454-Sequencing™ for the KEL, JR, and LAN Blood Groups.
Chapter number 11
Book title
Molecular Typing of Blood Cell Antigens
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2690-9_11
Pubmed ID
Book ISBNs
978-1-4939-2689-3, 978-1-4939-2690-9
Authors

Wieckhusen, Carola, Bugert, Peter, Carola Wieckhusen, Peter Bugert

Abstract

The KEL (ISBT 006), JR (ISBT 032), and LAN (ISBT 033) blood group systems are defined by a complex genetics with a large number of exons, and numerous gene variants. In order to sequence the 53 coding exons and flanking intron regions of all three blood group genes we developed a next-generation-sequencing method using 454-sequencing™ on a Genome Sequencer (GS) Junior™ system. Multiplex analysis of eight individual DNA samples was achieved using molecular identifiers.

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Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2015.
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