Chapter title |
Molecular RHD - RHCE Analysis by Multiplex PCR of Short Fluorescent Fragments
|
---|---|
Chapter number | 8 |
Book title |
Molecular Typing of Blood Cell Antigens
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2690-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2689-3, 978-1-4939-2690-9
|
Authors |
Yann Fichou, Claude Férec |
Abstract |
Several hundred variant alleles have been reported within the homologous RHD and RHCE genes that encode the antigens involved in the human Rh blood group system, which is of the main interest in the field of both transfusion and obstetrical medicine. Although these variants can be mostly characterized at the molecular level by sequence-specific primer polymerase chain reaction (SSP-PCR) and/or direct sequencing, some allelic combinations remain unresolved by conventional methods. Typically exon deletion or hybrid genes may be difficult to assess in a heterozygous context. Here we describe a qualitative and quantitative method to resolve copy number variations in the RH gene exons by quantitative multiplex polymerase chain reaction (PCR) of short fluorescent fragments (QMPSF). |
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