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Molecular Typing of Blood Cell Antigens

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Cover of 'Molecular Typing of Blood Cell Antigens'

Table of Contents

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    Book Overview
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    Chapter 1 Validation of Genotyping Protocols for Diagnostic Use
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    Chapter 2 High-resolution melting analysis of single nucleotide polymorphisms.
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    Chapter 3 High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping of Single Nucleotide Polymorphisms
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    Chapter 4 Blood Grouping Based on PCR Methods and Agarose Gel Electrophoresis
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    Chapter 5 Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry
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    Chapter 6 PCR with Sequence-Specific Primers for Typing of Diallelic Blood Groups
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    Chapter 7 High-resolution melting analysis for genotyping duffy blood group antigens.
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    Chapter 8 Molecular RHD - RHCE Analysis by Multiplex PCR of Short Fluorescent Fragments
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    Chapter 9 Microarrays in Blood Group Genotyping
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    Chapter 10 Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status.
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    Chapter 11 454-Sequencing™ for the KEL, JR, and LAN Blood Groups.
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    Chapter 12 Noninvasive Prenatal Blood Group Genotyping
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    Chapter 13 Genotyping of Human Platelet Antigens by BeadChip Microarray Technology
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    Chapter 14 Sequence-Based Typing for Platelet alloantigens
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    Chapter 15 Miniaturized Technology for DNA Typing: Cassette PCR
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    Chapter 16 Geno- and Phenotyping of Human Neutrophil Antigens
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    Chapter 17 Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
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    Chapter 18 Novel Approaches and Technologies in Molecular HLA Typing.
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    Chapter 19 Luminex-Based Methods in High-Resolution HLA Typing
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    Chapter 20 In Silico HLA Typing Using Standard RNA-Seq Sequence Reads.
Attention for Chapter 10: Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status.
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Chapter title
Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status.
Chapter number 10
Book title
Molecular Typing of Blood Cell Antigens
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2690-9_10
Pubmed ID
Book ISBNs
978-1-4939-2689-3, 978-1-4939-2690-9
Authors

Rieneck, Klaus, Clausen, Frederik Banch, Dziegiel, Morten Hanefeld, Klaus Rieneck, Frederik Banch Clausen, Morten Hanefeld Dziegiel

Abstract

The KEL1 antigen can give rise to immunization of KEL2 mothers. Maternal antibodies can be transferred to the fetus and destroy fetal red blood cells and their stem cell precursors and give rise to serious fetal disease. It is important to be able to predict the fetal KEL status in order to intervene in those pregnancies where the fetus is at risk, and to ascertain when the fetus is not at risk. Technically it can be demanding to predict KEL1 status from a maternal blood sample. The KEL1 allele is based on a single SNP present in about 1-10 % of cell-free maternal DNA after gestation week 10. Here we describe our protocol for antenatal prediction of fetal KEL1 status by NGS analysis of maternal DNA on a MiSeq instrument.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 25%
Student > Master 2 25%
Researcher 2 25%
Student > Ph. D. Student 1 13%
Professor > Associate Professor 1 13%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 5 63%
Biochemistry, Genetics and Molecular Biology 1 13%
Economics, Econometrics and Finance 1 13%
Agricultural and Biological Sciences 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2015.
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#18,412,793
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Outputs from Methods in molecular biology
#7,907
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#255,843
of 353,089 outputs
Outputs of similar age from Methods in molecular biology
#479
of 996 outputs
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