Chapter title |
Genotyping of Human Platelet Antigens by BeadChip Microarray Technology
|
---|---|
Chapter number | 13 |
Book title |
Molecular Typing of Blood Cell Antigens
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2690-9_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2689-3, 978-1-4939-2690-9
|
Authors |
Gerald Bertrand, Fabiana Conti, Bertrand, Gerald, Conti, Fabiana |
Abstract |
Human platelet antigen (HPA) typing plays a critical role in the diagnosis of fetal/neonatal alloimmune thrombocytopenia, and the prevention of posttransfusion purpura and refractoriness to platelet transfusions. The recent development of high-throughput genotyping methods, allowing simultaneous genotyping of as many as 17 HPAs, is of utmost interest for saving time and money. Here, we describe a microarray technology named "BeadChip," designed for HPA-1 to -9, -11, and -15 genotyping of up to 96 individuals, in approximately 5 h. This technology was used to study allele frequencies in Brazilian blood donors, considering the heterogeneous ethnic composition. |
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