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Mendeley readers
| Chapter title |
Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
|
|---|---|
| Chapter number | 17 |
| Book title |
Molecular Typing of Blood Cell Antigens
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2690-9_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2689-3, 978-1-4939-2690-9
|
| Authors |
Rudi Steffensen, John Baech, Kaspar R. Nielsen, Steffensen, Rudi, Baech, John, Nielsen, Kaspar R. |
| Abstract |
Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Researcher | 1 | 25% |
| Student > Master | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Medicine and Dentistry | 1 | 25% |
| Unknown | 1 | 25% |