| Chapter title |
Novel Approaches and Technologies in Molecular HLA Typing.
|
|---|---|
| Chapter number | 18 |
| Book title |
Molecular Typing of Blood Cell Antigens
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2690-9_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2689-3, 978-1-4939-2690-9
|
| Authors |
Dunn, Paul P J, Paul P. J. Dunn |
| Abstract |
The invention of the Polymerase Chain Reaction (PCR) has revolutionized molecular biology enabling gene isolation and characterization in hours rather than days. Scientists working in transplant diagnostics have proven to be pioneers in adapting this molecular technique to the clinical needs of histocompatibility testing. This chapter describes a number of novel genotyping technologies which have been used to address the challenges posed by genetic diversity seen in the extensive polymorphism in HLA genes. These novel approaches include single-stranded and duplex conformational analyses, real-time PCR, microarray hybridization, RNA-based sequencing, and the present day Next Generation Sequencing. The chapter concludes with a brief look at a possible next, Next Generation Sequencing system. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 2 | 20% |
| Other | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Student > Master | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Unspecified | 2 | 20% |
| Agricultural and Biological Sciences | 1 | 10% |
| Neuroscience | 1 | 10% |
| Unknown | 3 | 30% |