Chapter title |
In Silico HLA Typing Using Standard RNA-Seq Sequence Reads.
|
---|---|
Chapter number | 20 |
Book title |
Molecular Typing of Blood Cell Antigens
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2690-9_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2689-3, 978-1-4939-2690-9
|
Authors |
Boegel, Sebastian, Scholtalbers, Jelle, Löwer, Martin, Sahin, Ugur, Castle, John C, Sebastian Boegel, Jelle Scholtalbers, Martin Löwer, Ugur Sahin, John C. Castle, Castle, John C. |
Abstract |
Next-generation sequencing (NGS) enables high-throughput transcriptome profiling using the RNA-Seq assay, resulting in billions of short sequence reads. Worldwide adoption has been rapid: many laboratories worldwide generate transcriptome sequence reads daily. Here, we describe methods for obtaining a sample's human leukocyte antigen (HLA) class I and II types and HLA expression using standard NGS RNA-Seq sequence reads. We demonstrate the application using our algorithm, seq2HLA, and a publicly available RNA-Seq dataset from the Burkitt lymphoma cell line Raji. |
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