| Chapter title |
Label-Free Kinetic Analysis of an Antibody–Antigen Interaction Using Biolayer Interferometry
|
|---|---|
| Chapter number | 10 |
| Book title |
Protein-Protein Interactions
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2425-7_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
| Authors |
Sriram Kumaraswamy, Renee Tobias, Kumaraswamy, Sriram, Tobias, Renee |
| Abstract |
Biolayer Interferometry (BLI) is a powerful technique that enables direct measurement of biomolecular interactions in real time without the need for labeled reagents. Here we describe the analysis of a high-affinity binding interaction between a monoclonal antibody and purified antigen using BLI. A simple Dip-and-Read™ format in which biosensors are dipped into microplate wells containing purified or complex samples provides a highly parallel, user-friendly technique to study molecular interactions. A rapid rise in publications citing the use of BLI technology in a wide range of applications, from biopharmaceutical discovery to infectious diseases monitoring, suggests broad utility of this technology in the life sciences. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 81 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 19% |
| Student > Master | 14 | 17% |
| Researcher | 7 | 9% |
| Student > Doctoral Student | 5 | 6% |
| Student > Bachelor | 5 | 6% |
| Other | 7 | 9% |
| Unknown | 28 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 23% |
| Agricultural and Biological Sciences | 6 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 6% |
| Chemistry | 5 | 6% |
| Engineering | 5 | 6% |
| Other | 11 | 14% |
| Unknown | 30 | 37% |