Chapter title |
Using Peptide Arrays Created by the SPOT Method for Defining Protein-Protein Interactions
|
---|---|
Chapter number | 18 |
Book title |
Protein-Protein Interactions
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Yun Young Yim, Katherine Betke, Heidi Hamm, Yim, Yun Young, Betke, Katherine, Hamm, Heidi |
Editors |
Cheryl L. Meyerkord, Haian Fu |
Abstract |
Evaluating sites of protein-protein interactions can be an arduous task involving extensive mutagenesis work and attempts to express and purify individual proteins in sufficient quantities. Peptide mapping is a useful alternative to traditional methods as it allows rapid detection of regions and/or individual residues important for binding, and it can be readily applied to numerous proteins at once. Here we describe the use of the ResPep SL SPOT method to evaluate protein-protein binding interactions such as that between G-protein βγ subunits and SNARE proteins, identifying both regions of interest and subsequently individual residues which can then be manipulated in further biochemical assays to confirm their validity. |
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