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Chapter title |
Fluorescence Polarization Assay to Quantify Protein-Protein Interactions: An Update
|
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Chapter number | 19 |
Book title |
Protein-Protein Interactions
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Ronald T. Raines, Raines, Ronald T. |
Abstract |
A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 25% |
Researcher | 6 | 21% |
Student > Bachelor | 5 | 18% |
Student > Doctoral Student | 4 | 14% |
Professor | 2 | 7% |
Other | 3 | 11% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 32% |
Biochemistry, Genetics and Molecular Biology | 8 | 29% |
Chemistry | 6 | 21% |
Immunology and Microbiology | 1 | 4% |
Chemical Engineering | 1 | 4% |
Other | 2 | 7% |
Unknown | 1 | 4% |