Chapter title |
Studying Protein-Protein Interactions via Blot Overlay/Far Western Blot
|
---|---|
Chapter number | 24 |
Book title |
Protein-Protein Interactions
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Randy A. Hall, Hall, Randy A. |
Abstract |
Blot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiolabeled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as "Far Western blot." Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Czechia | 1 | 2% |
Germany | 1 | 2% |
Unknown | 42 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 20% |
Student > Postgraduate | 5 | 11% |
Student > Master | 5 | 11% |
Student > Bachelor | 4 | 9% |
Student > Ph. D. Student | 4 | 9% |
Other | 8 | 18% |
Unknown | 9 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 30% |
Biochemistry, Genetics and Molecular Biology | 13 | 30% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Physics and Astronomy | 1 | 2% |
Other | 3 | 7% |
Unknown | 10 | 23% |