You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Hexahistidine (6xHis) Fusion-Based Assays for Protein-Protein Interactions
|
---|---|
Chapter number | 23 |
Book title |
Protein-Protein Interactions
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Mary C. Puckett, Puckett, Mary C. |
Abstract |
Fusion-protein tags provide a useful method to study protein-protein interactions. One widely used fusion tag is hexahistidine (6xHis). This tag has unique advantages over others due to its small size and the relatively low abundance of naturally occurring consecutive histidine repeats. 6xHis tags can interact with immobilized metal cations to provide for the capture of proteins and protein complexes of interest. In this chapter, a description of the benefits and uses of 6xHis-fusion proteins as well as a detailed method for performing a 6xHis-pulldown assay are described. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 23% |
Student > Doctoral Student | 3 | 12% |
Student > Bachelor | 3 | 12% |
Researcher | 2 | 8% |
Student > Ph. D. Student | 2 | 8% |
Other | 2 | 8% |
Unknown | 8 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 35% |
Agricultural and Biological Sciences | 6 | 23% |
Environmental Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Chemistry | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 31% |